Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway
J. Clin. Invest. Igor Matushansky, et al. 117:3248 doi:10.1172/JCI31377 [
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Figure 2DKK1 and β-catenin patterns in hMSCs and MFH. (
A) ANOVA of gene expression analysis of the indicated sarcomas listed in order of genes overexpressed in MFH. (
B) DKK1 levels by RT-PCR in the indicated sarcomas. (
C) Dkk1 expression by IHC staining in the indicated sarcomas and percentage of samples expressing intense staining. Original magnification, ×200. (
D) Cell density of hMSCs proliferating in vitro in relation to β-catenin activity. Immunoblot analysis of Dkk1 and β-catenin in total cellular extracts (
E), nuclear extracts, (
F) and cytoplasmic extracts (
G) in the indicated samples. Immunoblot analysis of LEF1, c-myc, and cyclin D1 tubulin (
H) in the indicated samples.
