JCI - Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs)

Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs)

Carole Peyssonnaux, Annelies S. Zinkernagel, Reto A. Schuepbach, Erinn Rankin, Sophie Vaulont, Volker H. Haase, Victor Nizet, Randall S. Johnson

Published in Volume 117, Issue 7

J Clin Invest. 2007; 117(7):1926–1932 doi:10.1172/JCI31370

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Figure 2

Albumin-Cre/VHL^flox/flox mice develop erythrocytosis and iron deficiency.

(A) WT and Albumin-Cre/VHL^flox/flox (VHL^–/–) mice (4 weeks old). Right: Spleen and liver weights of 3- to 4-week-old WT and Albumin-Cre/VHL^flox/flox mice (n = 8 in each group). (B) H&E stainings of liver sections from WT and Albumin-Cre/VHL^flox/flox mice. Solid arrow indicates steatosis, dashed arrow inflammatory cell infiltrate. (C) EPO mRNA expression in kidney and liver of WT (black bars) and Albumin-Cre/VHL^flox/flox (gray bars) mice by real-time RT-PCR (n = 8). EPO, rbc, hematocrit, and hemoglobin levels in blood or serum from 5-week-old mice. n = 8 in each group. (D) Peripheral blood smears from WT and Albumin-Cre/VHL^flox/flox mice. Solid arrow indicates hypochromasia, dashed arrow anisocytosis. Right: mean corpuscular hemoglobin (MCH) of WT and Albumin-Cre/VHL^flox/flox mice. Original magnification, ×200. (E) Quantification of liver iron level in WT and Albumin-Cre/VHL^flox/flox mice using the method of Torrance et al. (33) (n = 5 in each group). (F) Western blot analysis of ferritin in liver extracts from WT and Albumin-Cre/VHL^flox/flox mice. (G) Iron staining of splenic sections by Perls Prussian blue. Original magnification, ×200.