Dominant-negative effect of the heterozygous C104R TACI mutation in common variable immunodeficiency (CVID)
J. Clin. Invest. Lilit Garibyan, et al. 117:1550 doi:10.1172/JCI31023 [
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Figure 3snIP analysis of TACI receptor assembly in ER microsomes. (
A and
B) Assembly of differentially tagged TACI monomers was examined in an in vitro translation system using ER microsomes. Membranes were solubilized with digitonin, and complexes were isolated by snIP using the protein C (PC) and SA-binding protein (SBP) affinity tags on the C terminus of the TACI chains. SBP-tagged complexes were captured by SA beads and PC tagged complexes were captured by anti-PC mAb. Asterisk (lane 6) represents mixing control in which TACI-SBP and TACI-PC monomers were translated separately and mixed prior to solubilization and IP.
