(A) Immunofluorescence staining for insulin (green) and glucagon (red) was performed in pancreatic sections from 10-week-old male WT mice (top), ob/ob littermates (middle), and adiponectin transgenic ob/ob mice. (B) Immunohistochemical staining of gonadal adipose tissue with anti-F4/80 antibodies suggests reduced infiltration of macrophages. Note that the difference in average adipocyte size between the 2 genotypes is apparent but less pronounced at this younger age. (C) FACS analysis of the stromal vascular fraction using F4/80 antibodies (left) and qRT-PCR for F4/80 (right) (n = 10 mice/group) were performed in gonadal WAT of adiponectin transgenic ob/ob mice and ob/ob littermates. Black curve shows control IgG staining; red curve shows anti-F4/80 staining. (D) mRNA levels of vWF and VCAM-1 from WAT were measured by qRT-PCR. A ratio of VCAM-1/vWF is provided as an indicator of local inflammation in the vascular endothelium. (E) Circulating IL-6 was measured in adiponectin transgenic ob/ob mice and ob/ob littermates (top); adipose tissue TNF-α message levels (bottom). (C–E) For these studies, 14-week-old male mice were used (n = 10 mice/group). *P < 0.05. Panels C–E were analyzed by Student’s t test.