(A) Changes in body weight were monitored (n = 15 mice/group). Representative animals are shown: an ob/ob mouse and an adiponectin transgenic ob/ob littermate at 12 weeks. (B) Body composition of 5-week-old mice was measured by MRI (n = 3 mice/group). (C) Body weight gain was tracked on normal chow or on a HF diet in 10-week-old adiponectin transgenic ob/ob mice and ob/ob littermates. (D) Sections of gonadal WAT and (E) pancreatic islets from adiponectin transgenic ob/ob mice (top) and ob/ob littermates (bottom) were H&E stained. The average adiponectin size calculated (D, right panel) and surface area of >100 islets stained for insulin was measured and average islet area is indicated (E, right panel). (F) Frozen sections of liver from adiponectin transgenic ob/ob mice and ob/ob littermates were stained with oil red O (left panel). Liver triglyceride content (middle panel) and DAG levels (right panel) were analyzed. (G) Intraabdominal visceral fat pads from WT mice, adiponectin transgenic ob/ob mice, and ob/ob littermates were weighed and presented as percentage of total body weight (F and G, n = 4–5 mice/group). (H) Pericardial fat pads from 10-week-old female mice were imaged by MRI and reconstituted into a 3D representation. A representative example is shown. (I) Mice were fasted for 6 hours and injected with 1 mU of insulin/g body weight. At indicated time points, 1 mouse was sacrificed and liver protein was extracted and analyzed for the total and phosphorylated forms of the indicated targets using 3 independent measurements for each time point. *P < 0.05; **P < 0.01. Panels A and C were analyzed by ANOVA; panels D, F, G, and I were analyzed by Student’s t test.