(A) Circulating glucose and insulin levels measured during OGTT. (B) Plasma triglyceride (TG) levels measured during lipid challenge in adiponectin transgenic ob/ob mice and their ob/ob littermates. (C) Plasma triglyceride levels were measured during a lipid challenge in adiponectin transgenic ob/ob mice after an injection of 2 monoclonal anti-adiponectin antibodies (Mono-Ab) or control mouse IgGs (CTRL-IgG). (D) Plasma insulin (right) and FFA levels (left) monitored after i.p. CL-316,243 injection in adiponectin transgenic ob/ob mice and their ob/ob littermates. (E) Cholesterol and triglyceride levels were measured in plasma samples subfractionated by gel filtration chromatography. IDL, intermediate density lipoprotein. (F) apoE levels were measured in the same fractions by Western blot analysis (top) and quantitated (bottom). (G) Adiponectin transgenic ob/ob mice and their ob/ob littermates were fed normal chow (N chow) or high-fat diet (HF) for 6 weeks. Fasting glucose levels (top left), glucose levels during an OGTT (top right), insulin levels (bottom left), and FFA levels (bottom right) were determined in adiponectin transgenic ob/ob mice and their ob/ob littermates (A–G, n = 5 mice/group). (H) 2DG was centrally delivered to adiponectin transgenic ob/ob mice, ob/ob littermates, and WT mice, and blood glucose levels were monitored during indicated times (n = 3 mice/group). Experiments were performed with 10- to 12-week-old adiponectin transgenic ob/ob mice, ob/ob littermates, and WT male mice. *P < 0.05; **P < 0.01. Panels A and B were analyzed by ANOVA; panels D and G were analyzed by Student’s t test.