Figure 1
ob/ob mice overexpressing adiponectin display physiological overexpression of adiponectin in circulation with normal adiponectin complex distribution.
(A) Circulating adiponectin levels were measured by RIA in 12-week-old adiponectin transgenic ob/ob mice (Ad Tg ob/ob), their ob/ob littermates, and ob/ob mice with PPARγ agonist treatment (n = 5 mice/group). (B) Tissue levels of adiponectin were measured in various fat pads, determined by Western blot analysis, and normalized with GDI in 12-week-old male adiponectin transgenic ob/ob mice, their ob/ob littermates, and ob/ob mice with PPARγ agonist treatment (n = 5 mice/group). (C) The complex distribution of adiponectin of female mice was determined using gel filtration chromatography of serum samples followed by Western blot analysis of adiponectin in different fractions. A representative sample is shown. HMW, high molecular weight form; LMW, low molecular weight form (hexamer). *P < 0.05; **P < 0.01 by Student’s t test.
