Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas
J. Clin. Invest. Grzegorz Sarek, et al. 117:1019 doi:10.1172/JCI30945 [
Go to this article.]
Figure 6Nutlin-3a disrupts the p53-MDM2-LANA interaction. (
A) Extracts from the BC-3 cell line exposed for 12 hours to vehicle control or 7 μM Nutlin-3a were separated using gel filtration chromatography. Fractions were analyzed by Western blotting with antibodies against p53, MDM2, and LANA. The elution profile of molecular weight standards is indicated above the lanes. (
B) The peak fractions for LANA (asterisks in
A) from BC-3 cells treated with either vehicle control or 7 μM Nutlin-3a for 12 hours were used in immunoprecipitations with anti-p53 or anti-MDM2 antibodies. As a control, a duplicate sample from the same fraction was immunoprecipitated with mouse IgG. Immunocomplexes were resolved by SDS-PAGE and analyzed by Western blotting with antibodies against p53, MDM2, and LANA.
