Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas
J. Clin. Invest. Grzegorz Sarek, et al. 117:1019 doi:10.1172/JCI30945 [
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Figure 5DNA damage signaling enhances the cytotoxic effect of Nutlin 3a. (
A) BC-3 and CZE cells were immunostained with antibody against γH2AX, the phosphorylated form of histone H2AX. The nuclear morphology was visualized by Hoechst staining. (
B) Percentage of γH2AX-positive cells in PEL cells (BC-1, BC-3, and BCBL-1) and EBV-transformed LCLs (CZE and IHE). Results represent the mean of 2 independent experiments. (
C) BC-1, BC-3, BCBL-1, CZE, IHE, DG-75, and HL-60 cells were incubated for 12 hours in the presence or absence of 7 μM Nutlin-3a. Whole-cell lysates were subjected to SDS-PAGE followed by Western blotting and analyzed for phosphorylated Chk2(Thr68) and total Chk2 expression. (
D) Survival curves for BC-1, IHH, and IHE cells (some gamma-irradiated at 1 Gy; IR) incubated with Nutlin-3a in the presence or absence of caffeine (2 mM). Cell death was determined by trypan blue exclusion at 24, 48, and 72 hours after treatment. Values represent the percentage of viable cells relative to that of DMSO control.
