Figure 4
Nutlin-3a selectively kills KSHV-infected cells.
(A) U2OS and EA.hy 926 cells in the absence or presence of latent rKSHV infection were treated with 7 μM Nutlin-3a, and cell death was assessed by trypan blue exclusion at 24, 48, and 96 hours of treatment. Values represent the percentage of dead cells induced by Nutlin-3a treatment. The percentage of dead cells in the DMSO control was subtracted as a background. Each value represents the mean of 3 independent experiments. (B) Noninfected and rKSHV-infected EA.hy 926 cells were incubated for 12 hours in the presence or absence of 7 μM Nutlin-3a. Whole-cell lysates were subjected to SDS-PAGE followed by Western blotting and analyzed for p53 and MDM2 expression. Actin immunoblot is shown as a loading control.
