Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas
J. Clin. Invest. Grzegorz Sarek, et al. 117:1019 doi:10.1172/JCI30945 [Go to this article.]

Figure 3
Nutlin-3a has cytotoxic activity in PEL cells. (A) PEL cell lines (BC-1, BC-3, and BCBL-1) and KSHV-infected LCL IHH (green symbols), EBV-transformed LCLs (CZE and IHE; blue symbols), or mutant p53 cells (DG-75 and HL-60; red symbols) were cultured for 5 days with 7 μM Nutlin-3a. Cell viability was determined by trypan blue exclusion at the indicated time points. Results are shown as survival curves denoting percentage of viable cells relative to the vehicle control. Data represent the mean of 3 independent experiments. (B) Scatter plot of annexin V–FITC/PI flow cytometry of BC-1 cells after exposure to 7 μM of Nutlin-3a or vehicle control for different time periods. (C) Apoptosis in BC-3, BCBL-1, CZE, IHE, DG-75, and HL-60 cells was assessed at 96 hours after treatment with 7 μM Nutlin-3a or vehicle control by annexin V–FITC/PI binding and measured by flow cytometry analysis. Lower left quadrants represent viable cells (annexin V– and PI-negative); lower right quadrants represent early apoptotic cells (annexin V–positive, PI-negative) demonstrating cytoplasmic membrane integrity; upper right quadrants represent nonviable, late apoptotic cells (annexin V– and PI-positive). Numbers indicate the percentage of cells in each quadrant. Shown is 1 representative experiment of 3.