Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas
J. Clin. Invest. Grzegorz Sarek, et al. 117:1019 doi:10.1172/JCI30945 [Go to this article.]

Figure 1
Nutlin-3a activates p53 and its target genes in PEL cells. KSHV-infected PEL cells (BC-1, BC-3, and BCBL-1), EBV-transformed LCLs (CZE and IHE), and cells with mutant p53 (DG-75 and HL-60) were incubated for 12 hours in the presence (+) or absence (–) of 7 μM Nutlin-3a. Whole-cell lysates were subjected to SDS-PAGE followed by Western blotting and analyzed for p53, MDM2, phosphorylated-p53(Ser15) [p-p53(Ser15)], 53BP1, p21^CIP1, and Bax expression. Actin immunoblot is shown as a loading control.