Constitutive IKK2 activation in acinar cells is sufficient to induce pancreatitis in vivo
J. Clin. Invest. Bernd Baumann, et al. 117:1502 doi:10.1172/JCI30876 [Go to this article.]

Figure 2
Cerulein-induced pancreatitis is ameliorated in Ela.rtTA×IKK2-DN mice. (A) Experimental design. Mice were pretreated with Dox 24 hours before induction of pancreatitis through repeated cerulein injections. (B) Morphological examination of Ela.rtTA×IKK2-DN and single-transgenic IKK2-DN controls revealed comparable histology 24 hours after Dox injection (0 hours). Whereas cerulein induced pancreatitis in controls 6 and 18 hours after starting cerulein injections, this phenotype was reduced in Ela.rtTA×IKK2-DN mice. Scale bars: 50 μm. (C) Serum lipase values were reduced in Ela.rtTA×IKK2-DN compared with controls 6 and 18 hours after starting cerulein injections. (D) Histological score improved in Ela.rtTA×IKK2-DN mice compared with controls during the observation period. (E) Immunoblot confirmed overexpression of IKK2-DN in Ela.rtTA×IKK2-DN mice compared with controls. ERK2 served as a loading control.