Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice
J. Clin. Invest. Marco B. Rust, et al. 117:1708 doi:10.1172/JCI30630 [
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Figure 3Hematologic characterization of
Kcc-KO mice.
(
A) rbc densities determined by centrifugation through phthalate mixtures of defined density. (
B) rbc densities determined by centrifugation through discontinuous Stractan gradients. FI, fraction I. (
C) Distinct rbc bands were collected and the rbc numbers quantified to evaluate relative number of rbc per fraction.
Kcc1–/– and
Kcc3–/– rbc densities were indistinguishable from WT, but
Kcc1–/–Kcc3–/– rbc densities were reduced. (
D) Osmotic lysis curve shows significantly increased osmotic sensitivity only for rbc lacking both KCCs. *
P ≤ 0.05; **
P ≤ 0.005.
