Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice
J. Clin. Invest. Marco B. Rust, et al. 117:1708 doi:10.1172/JCI30630 [Go to this article.]

Figure 2
K-Cl cotransport activity in rbc of WT and KO mice. K-Cl cotransport was determined by measuring K⁺ efflux in the presence or absence of Cl^– (replaced with SFA^–) in isotonic or hypotonic conditions and in the presence of staurosporine (1 μM) or urea (500 mM) added to the isotonic solution. Gray bars indicate a significant difference between stimulated and isotonic K⁺ fluxes in the presence of Cl^–. Black bars indicate a significant difference between K⁺ fluxes in a single condition in the presence and absence of Cl^–. WT K-Cl cotransport activity (A) was unaltered in rbc of Kcc1^–/– mice (B). (C, D, and E) Disruption of Kcc3 reduced K-Cl cotransport activity in all conditions examined, with an apparent gene-dosage effect. (F) In rbc of Kcc1^–/–Kcc3^–/– mice, equivalent K⁺ fluxes in the presence and absence of Cl^– indicated complete loss of K-Cl cotransport activity. Numbers in parentheses refer to the number of independent samples in each category.