Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice
J. Clin. Invest. Marco B. Rust, et al. 117:1708 doi:10.1172/JCI30630 [
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Figure 2K-Cl cotransport activity in rbc of WT and KO mice. K-Cl cotransport was determined by measuring K
+ efflux in the presence or absence of Cl
– (replaced with SFA
–) in isotonic or hypotonic conditions and in the presence of staurosporine (1 μM) or urea (500 mM) added to the isotonic solution. Gray bars indicate a significant difference between stimulated and isotonic K
+ fluxes in the presence of Cl
–. Black bars indicate a significant difference between K
+ fluxes in a single condition in the presence and absence of Cl
–. WT K-Cl cotransport activity (
A) was unaltered in rbc of
Kcc1–/– mice (
B). (
C,
D, and
E) Disruption of
Kcc3 reduced K-Cl cotransport activity in all conditions examined, with an apparent gene-dosage effect. (
F) In rbc of
Kcc1–/–Kcc3–/– mice, equivalent K
+ fluxes in the presence and absence of Cl
– indicated complete loss of K-Cl cotransport activity. Numbers in parentheses refer to the number of independent samples in each category.