Mice lacking the signaling molecule CalDAG-GEFI represent a model for leukocyte adhesion deficiency type III
J. Clin. Invest. Wolfgang Bergmeier, et al. 117:1699 doi:10.1172/JCI30575 [
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Figure 3CalDAG-GEFI deficiency causes impaired β
1 integrin– and β
2 integrin–mediated adhesion of neutrophils in vitro.
(
A and
B) Neutrophil adhesion to fibronectin (
A) or fibrinogen (
B) in vitro. WT or CalDAG-GEFI–deficient neutrophils isolated from bone marrow were added to fibronectin- or fibrinogen-coated plates and incubated for 30 minutes in the presence or absence (i.e., resting) of 300 nM LTB
4 or 3 or 30 nM PAF. Plates were washed and adherent neutrophils were counted.
n = 4. *
P < 0.05, **
P < 0.01, ***
P < 0.001.
