Mice lacking the signaling molecule CalDAG-GEFI represent a model for leukocyte adhesion deficiency type III
J. Clin. Invest. Wolfgang Bergmeier, et al. 117:1699 doi:10.1172/JCI30575 [Go to this article.]

Figure 2
Impaired Rap1 activation in CalDAG-GEFI–deficient neutrophils. Western blots of affinity-precipitated Rap1-GTP showing strongly decreased Rap1 activation in CalDAG-GEFI–deficient neutrophils (–/–) stimulated with LTB₄ (300 nM for 30 seconds), C5a (75 ng/ml for 30 seconds), or PAF (3 or 30 nM for 30 seconds) relative to that of WT neutrophils (+/+). Equivalent loading of GTP onto Rap1 in neutrophils from WT and CalDAG-GEFI^–/– mice was shown by preincubation of lysates with GTP-γS. Total Rap1 levels were determined in whole cell lysates of WT and CalDAG-GEFI–deficient neutrophils. Results are representative of 3 individual experiments.