Mice lacking the signaling molecule CalDAG-GEFI represent a model for leukocyte adhesion deficiency type III
J. Clin. Invest. Wolfgang Bergmeier, et al. 117:1699 doi:10.1172/JCI30575 [Go to this article.]

Figure 1
Degranulation, calcium flux, and ROS production in stimulated neutrophils. (A) Mac-1 expression. PBLs from WT and CalDAG-GEFI^–/– mice were kept resting or were activated for 10 minutes with LTB₄ (300 nM), C5a (50 ng/ml), or PMA (200 nM), stained with antibodies against Mac-1, and immediately analyzed by flow cytometry. n = 6. (B) Calcium flux. PBLs from WT and CalDAG-GEFI^–/– mice were loaded with the calcium-sensitive dye Fluo-3, activated with the indicated doses of LTB₄ or C5a, and immediately analyzed by flow cytometry. Results are representative of 6 individual experiments. (C) ROS formation. PBLs from WT and CalDAG-GEFI^–/– mice were loaded with the ROS-sensitive agent DCFDA, activated with PMA (2 μM) or fMLP (5 μM) for 30 minutes at 37°C, and immediately analyzed by flow cytometry. n = 5. ROS production is expressed as fold increase of mean fluorescence intensity over unstimulated cells. No significant differences in Mac-1 expression, calcium flux, or ROS production were observed between WT and CalDAG-GEFI–deficient neutrophils.