Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance
J. Clin. Invest. Li Liu, et al. 117:1679 doi:10.1172/JCI30565 [Go to this article.]

Figure 2
Transgenic mice with DGAT1 overexpression in skeletal muscle. (A) The Dgat1 transgene contains (from the 5′ end to the 3′ end) the 3.3-kb MCK promoter, a human Dgat1 cDNA containing its own initiation and termination codons, and the genomic sequence of human growth hormone (hGH) containing the last 3 exons and 2 introns as indicated. (B) Tissue distribution of the Dgat1 transgene mRNA levels measured by reverse transcription and PCR amplification. S. intestine, small intestine; gastro., gastrocnemius. (C) RT-PCR quantification of total DGAT1 and DGAT2 mRNA levels in soleus muscles of WT and transgenic mice (n = 5 in each group). (D) Total DGAT activity levels in membrane fractions of soleus muscles from WT and transgenic mice (n = 5 in each group). **P < 0.01.