Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance
J. Clin. Invest. Li Liu, et al. 117:1679 doi:10.1172/JCI30565 [Go to this article.]

Figure 1
Upregulation of myocellular DGAT increases intracellular TG levels and decreases intracellular DAG levels. (A–C) Overexpression of DGAT1 via recombinant adenovirus (Dgat1) in differentiated C2C12 myocytes, showing DGAT activity levels (A), TG content (B), and DAG level (C) compared with mock-transduced cells or cells transduced with a control recombinant adenovirus expressing GFP. (D–G) Exercise-induced DGAT activation (D) and changes in myocellular TG (E), DAG (F), and ceramide (G) levels in isolated soleus muscles from 3-month-old male mice after 1 week swimming training (Swim; n = 6) as compared with age-, gender-, and diet-matched sedentary mice (Ctrl; n = 4). *P < 0.05; **P < 0.01.