Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance
J. Clin. Invest. Li Liu, et al. 117:1679 doi:10.1172/JCI30565 [
Go to this article.]

Figure 1Upregulation of myocellular DGAT increases intracellular TG levels and decreases intracellular DAG levels. (
A–
C) Overexpression of DGAT1 via recombinant adenovirus (Dgat1) in differentiated C2C12 myocytes, showing DGAT activity levels (
A), TG content (
B), and DAG level (
C) compared with mock-transduced cells or cells transduced with a control recombinant adenovirus expressing GFP. (
D–
G) Exercise-induced DGAT activation (
D) and changes in myocellular TG (
E), DAG (
F), and ceramide (
G) levels in isolated soleus muscles from 3-month-old male mice after 1 week swimming training (Swim;
n = 6) as compared with age-, gender-, and diet-matched sedentary mice (Ctrl;
n = 4). *
P < 0.05; **
P < 0.01.