Thrombin-initiated platelet activation in vivo is vWF independent during thrombus formation in a laser injury model
J. Clin. Invest. Christophe Dubois, et al. 117:953 doi:10.1172/JCI30537 [Go to this article.]

Figure 6
Role of thrombin, vWF, and GPVI in platelet accumulation and calcium mobilization. WT, vWF^–/–, or FcRγ^–/– platelets loaded with fura-2/AM were infused into a recipient mouse of the same genotype. In some experiments, the WT recipient mouse was treated with lepirudin. Thrombus formation was performed as described in Figure 2 and fura-2–loaded platelets imaged for platelet accumulation and calcium mobilization. Representative images were obtained before injury (pre) and 30, 60, 90, 120, and 150 seconds after injury.