Thrombin-initiated platelet activation in vivo is vWF independent during thrombus formation in a laser injury model
J. Clin. Invest. Christophe Dubois, et al. 117:953 doi:10.1172/JCI30537 [
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Figure 4In vivo imaging of calcium mobilization during platelet activation during thrombus formation in
vWF–/– mice.
(
A) Platelets isolated from
vWF–/– mice were loaded with fura-2/AM. Platelets (250 × 10
6 to 300 × 10
6 cells) were infused into the circulation of a
vWF–/– mouse. Following laser-induced vessel wall injury, thrombus formation was observed and images recorded over time. (
A) Representative composite images of fluorescence and bright-field data depicting thrombus formation show fura-2–loaded platelet accumulation (green, yellow) and calcium mobilization (yellow) in a
vWF–/– mouse. (
B) Calcium mobilization during thrombus formation represented by the median integrated fluorescence intensity (
y axis) of fura-2–labeled platelets excited at 340 nm. Black: WT mice; blue:
vWF–/– mice.
