Thrombin-initiated platelet activation in vivo is vWF independent during thrombus formation in a laser injury model
J. Clin. Invest. Christophe Dubois, et al. 117:953 doi:10.1172/JCI30537 [Go to this article.]

Figure 2
In vivo imaging of calcium mobilization during platelet activation during thrombus formation in WT mice. Platelets isolated from WT mice were loaded with fura-2/AM. Platelets (250 × 10⁶ to 300 × 10⁶ cells) were infused into the circulation of a WT mouse. Following laser-induced vessel wall injury, thrombus formation was observed and images recorded over time. (A) Representative composite images of fluorescence and bright-field data depicting thrombus formation show fura-2–loaded platelet accumulation (green, yellow) and calcium mobilization (yellow). Pre, before injury. (B) Platelet accumulation during thrombus formation represented by the median integrated fluorescence intensity (y axis) of fura-2–labeled platelets excited at 380 nm. Platelets were studied in the absence (upper curve) or presence (lower curve) of BAPTA-AM. F platelets, median integrated fluorescence of platelets, with excitation at 380 nm and emission at 510 nm. (C) Representative composite images of fluorescence and bright-field data depicting thrombus formation show fura-2–loaded platelet accumulation (green, yellow) and calcium mobilization (yellow) in the presence of BAPTA-AM. (D) Calcium mobilization during thrombus formation depicted by the median integrated fluorescence intensity (y axis) of fura-2–labeled platelets excited at 340 nm. Platelets were studied in the absence (upper curve) or presence (lower curve) of BAPTA-AM. These data represent the median of experiments performed in 18 thrombi from 3 mice. F calcein, median integrated fluorescence of the signal corresponding to calcein-labeled platelets.