CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease
J. Clin. Invest. Nicolas Barnich, et al. 117:1566 doi:10.1172/JCI30504 [Go to this article.]

Figure 3
CEACAM6 expression supports AIEC adhesion. (A) Western blot analysis of whole protein extracts from ileal biopsies taken in both involved and uninvolved areas of 9 CD patients and from 9 ileal biopsies from controls using anti-CEACAM5, anti-CEACAM6, and anti–β-actin antibodies. (B) Western blot analysis of whole protein extracts from colonic biopsies from 3 controls, 3 UC patients, and 3 CD patients using anti-CEACAM6 and anti–β-actin antibodies. (C) Adhesion indices of AIEC strain LF82 to the brush border of enterocytes from CD patients in the presence of monoclonal antibodies raised against CEACAM1, CEACAM5, CEACAM6, THP, and CD48. (D) Adhesion indices of AIEC strain LF82 to the brush border of enterocytes from 4 CD patients in the presence of anti-CEACAM6 monoclonal antibody. (E) Confocal microscopic analysis of HeLa cells transfected with human CEACAM6 cDNA cloned and infected with AIEC LF82 harboring a GFP construct. Original magnification, ×1,000. CEACAM6 expression was detected using anti-CEACAM6 monoclonal antibody and a Texas red–conjugated anti-mouse IgG. Arrows show clear colocalization (yellow staining) between CEACAM6 and bacteria.