CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease
J. Clin. Invest. Nicolas Barnich, et al. 117:1566 doi:10.1172/JCI30504 [Go to this article.]

Figure 2
Type 1 pili–mediated adhesion to the brush border of ileal enterocytes. (A) Adhesion of the LF82-derived type 1 pili–negative mutant 52D11 (fimA::Tn5phoA) to the brush border of enterocytes isolated from CD patients and controls. (B) Adhesion of AIEC LF82 to the brush border of enterocytes isolated from CD patients and controls in the absence or presence of 2% d-mannose. (C) Adhesion of LF82 bacteria expressing type 1 pili variant and of nonpathogenic E. coli K-12 transformed with pPil38 to induce the expression of K-12 type 1 pili (left panel) or of LF82 bacteria expressing either LF82 type 1 pili variant or K-12 type 1 pili (middle panel) to the brush border of enterocytes isolated from CD patients. Western blot analysis using anti–type 1 pili antibodies performed on whole bacterial extracts showed similar amounts of type 1 pili expressed by the various strains (right panel). (D) Expression of a highly mannosylated molecule on the brush border of ileal enterocytes isolated from CD patients. Phase contrast microscopy and fluorescence labeling using ConA-FITC of ileal enterocytes from a CD patient and a control. Original magnification, ×400.