Crosstalk between G_i and G_q/G_s pathways in airway smooth muscle regulates bronchial contractility and relaxation
J. Clin. Invest. 117:5 doi:10.1172/JCI30489 [Go to this article.]

Figure 4
G_αi2-OE or G_αi2-IP in mouse ASM alters responsiveness to M₃-muscarinic and β₂AR agonists.

(A) Intact, ventilated mice were administered the indicated doses of inhaled methacholine and airway resistance measured. The G_αi2-IP mice had a greater response to methacholine than NTG mice, while G_αi2-OE mice were less responsive than NTG. ^†P < 0.005 versus NTG, ANOVA. (B) Mice were pretreated with vehicle or inhaled isoproterenol and then underwent the methacholine challenge. NTG mice showed the expected decrease in maximal contraction while the G_αi2-OE mice showed no statistical difference in airway resistance with isoproterenol. The G_αi2-IP mice had an enhanced response to isoproterenol. Results are from 6–8 independent experiments. *P < 0.05; ^#P < 0.01.