Inherited human cPLA2α deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction
J. Clin. Invest. David H. Adler, et al. 118:2121 doi:10.1172/JCI30473 [
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Figure 4Ex vivo TxB
2 production by washed platelets after the addition of deuterated AA (2 μM).
Endogenous substrate converted by platelet COX-1 was measured as nondeuterated TxB
2, whereas exogenous substrate was measured as deuterated TxB
2. Both deuterated and nondeuterated TxB
2 were measured simultaneously in the same samples after addition of deuterated AA to assess endogenous AA release triggered by exogenous AA. Error bars represent ± SD.
