Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient mice
J. Clin. Invest. Noriaki Okamoto, et al. 117:997 doi:10.1172/JCI30379 [Go to this article.]

Figure 9
Decreased expression of chemokine receptors on lymphocytes in aLNs (transplanted into SCID mice after the first immunization) by i.v. injection of NP-OVA. (A) CCR7 and CXCR5 expression FACS profiles on lymphocytes in aLNs of recipient SCID or naive BALB/c mice. (B) Changes in chemokine receptor RNA levels as assessed by quantitative RT-PCR in aLNs of recipient SCID or naive BALB/c mice after antigen stimulation. –, no antigen stimulation; +, immunization with NP-OVA (100 μg/mouse). The y axis shows numbers of cells (A) and the relative RNA levels (B). The expression of each receptor RNA level was normalized to the expression of glyceradehyde-3-phosphate dehydrogenase (gapdh). *P < 0.1.