Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient mice
J. Clin. Invest. Noriaki Okamoto, et al. 117:997 doi:10.1172/JCI30379 [Go to this article.]

Figure 6
Enrichment of memory-type (CD44^hiCD62L^lo) CD4⁺ T cells in aLNs or spleens of aLN-transplanted SCID mice. (A) FACS plots demonstrating an increase in the CD44^hiCD62L^lo memory-type CD4⁺ T cell population in aLNs compared with that in lymph nodes of recipient BALB/c mice. Downward pointing arrows indicate the CD44/CD62L profile of cells within the CD4⁺ T cell gate. (B) FACS plots demonstrating an increase in the CD44^hiCD62L^lo memory-type CD4⁺ T cell population in the spleens of aLN-transplanted SCID mice, regardless of antigen stimulation, compared with that of lymph nodes or spleens in normal BALB/c mouse preimmunized with the same doses of NP-OVA. Numbers in the plots in A and B indicate the percentage of cells within the CD62L^lo/CD44⁺ gate. (C) Immunohistochemical staining of CD127⁺ T cells in aLNs. Three weeks after aLNs were formed in NP-OVA preimmunized BALB/c mice, aLNs and lymph nodes of recipient BALB/c mice were stained with FITC-labeled anti-mouse CD3, anti-mouse CD127 antibodies, and Alexa Fluor 594–conjugated anti-rat IgG. Most CD3⁺ T cells were also CD127⁺.