Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient mice
J. Clin. Invest. Noriaki Okamoto, et al. 117:997 doi:10.1172/JCI30379 [Go to this article.]

Figure 4
Increased number of IgG1 NP-specific AFCs in aLN-transplanted SCID mice after immunization with NP-OVA. (A and B) Number of NP-specific IgG AFCs in aLNs (A) or spleens (B) of aLN-transplanted SCID mice. Mice were immunized i.v. with NP-OVA on days 1 and 7 (100 μg/mouse and 10 μg/mouse, respectively) after transplantation of aLNs into SCID mice on day 0. The number of lymphocytes in spleens of aLN-transplanted SCID mice before and after NP-OVA immunization is denoted by the line (B). (C) Upon the second antigen stimulation, large numbers of IgG1 NP-specific AFCs were also detected in the BM of aLN-transplanted SCID mice. (D) Number of NP-specific IgG AFCs in spleens of recipient SCID and BALB/c mice. (E and F) Number of NP-specific IgG AFCs in aLNs (E) or spleens (F) of aLN-transplanted SCID mice immunized i.v. with NP-OVA on days 7 and 14 (100 μg/mouse and 10 μg/mouse, respectively) after transplantation of aLNs into SCID mice on day 0. The number of lymphocytes in spleens of aLN-transplanted SCID mice before and after NP-OVA immunization is denoted by the line (F).