Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient mice
J. Clin. Invest. Noriaki Okamoto, et al. 117:997 doi:10.1172/JCI30379 [Go to this article.]

Figure 3
Kinetics of aLN formation. Immunohistochemical staining of the cells in TEL-2–LTα stromal cell–embedded collagen sponges in BALB/c mice. The time course of aLN formation was examined 3, 9, 13, and 18 days after inoculation of collagen sponge into renal subcapsular space in BALB/c mice. Antibodies were as follows: FITC-labeled anti-mouse CD3, anti-mouse FDC-M1, and Alexa Fluor 594–conjugated anti-rat IgG (A–D); FITC-labeled anti-mouse B220, anti-mouse FDC-M1, and Alexa Fluor 594–conjugated anti-rat IgG (E–H).