Involvement of PLEKHM1 in osteoclastic vesicular transport and osteopetrosis in incisors absent rats and humans
J. Clin. Invest. Liesbeth Van Wesenbeeck, et al. 117:919 doi:10.1172/JCI30328 [
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Figure 7Localization of Plekhm1 to endosomes is dependent on prenylated, active Rab7. HEK293 cells were incubated for 24 hours in the presence of the Rab GGTase inhibitor 3-PEHPC (1 mM) immediately following transfection with EGFP-Rab7 and Plekhm1-dsRedM. (
A and
B) The vesicular localization of both Rab7 and Plekhm1 (
A) was disrupted by 3-PEHPC (
B). (
C) Acidic vesicles were stained with lysotracker (LT) prior to fixation. No effect of 3-PEHPC on the integrity of the endosomal/lysosomal compartment was observed. (
D and
E) HEK293 cells were transfected with Plekhm1-dsRedM and constitutively active EGFP–Rab7-Q67L (
D) or dominant-negative EGFP–Rab7-T22N (
E). Plekhm1 and Rab7-Q67L colocalized on intracellular vesicles, whereas Rab7-T22N and plekhm1 were both diffusely distributed throughout the cytoplasm. Panels represent 1-μm
xy optical sections. Scale bars: 10 μm.
