Involvement of PLEKHM1 in osteoclastic vesicular transport and osteopetrosis in incisors absent rats and humans
J. Clin. Invest. Liesbeth Van Wesenbeeck, et al. 117:919 doi:10.1172/JCI30328 [Go to this article.]

Figure 6
Subcellular localization of Plekhm1 in HEK 293 cells. HEK293 cells were transfected with plasmids, then fixed 24 hours later and analyzed by confocal microscopy. (A) Both Plekhm1-EGFP and Plekhm1-dsRedM displayed a diffuse cytoplasmic distribution, but were also clearly associated with intracellular vesicles, which frequently became abnormally enlarged. (B–E) HEK293 cells were cotransfected with Plekhm1-dsRedM and EGFP-Rab5 (B), EGFP-Rab6 (C), EGFP-Rab7 (D), or EGFP-Rab9 (E). Plekhm1 did not colocalize with either Rab5 or Rab6, but partially colocalized with Rab9 and completely colocalized to intracellular vesicles with Rab7. Interestingly, Plekhm1 showed more pronounced localization to these Rab7-positive vesicles when cotransfected with EGFP-Rab7. (F and G) HEK293 cells were transfected with EGFP-Rab7 and Plekhm1-dsRedM mutants corresponding to the mutations found in the osteopetrotic patient (hplekhm1; F) and the ia rat (rplekhm1; G). Neither mutant form of Plekhm1 colocalized with Rab7. Panels represent 1-μm xy optical sections. Scale bars: 10 μm.