Involvement of PLEKHM1 in osteoclastic vesicular transport and osteopetrosis in incisors absent rats and humans
J. Clin. Invest. Liesbeth Van Wesenbeeck, et al. 117:919 doi:10.1172/JCI30328 [Go to this article.]

Figure 4
Characterization of the osteoclasts from the family with autosomal-recessive osteopetrosis. Cells were cultured on dentine discs (A–D) or plastic (E and F) in the presence of RANKL for 10 days and then fixed, stained, and analyzed by confocal microscopy (A–D) or conventional microscopy (E and F). (A) Staining of F-actin with FITC-phalloidin (green stain), acidic vesicles with lysotracker (red stain), and osteoclast membrane with anti-VNR antibodies (blue stain). (B) Staining of the dentine surface with FL-ALN (green stain) and F-actin with TRITC-phalloidin (red stain). Dark areas correspond to resorption pits (asterisks). (C) Staining of the dentine surface with FL-ALN and nuclei with Sytox Green (green); F-actin with TRITC-phalloidin (red); osteoclast membrane with anti-VNR antibodies (blue). A–C represent 1-μm xy optical sections; D represents zx reconstructions of osteoclasts in C. Scale bar: 10 μm. (E and F) Staining for TRAP in osteoclasts. Original magnification, ×10 (E) and ×40 (F).