JCI - The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis

The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis

Claudia Scholl, Dimple Bansal, Konstanze Döhner, Karina Eiwen, Brian J.P. Huntly, Benjamin H. Lee, Frank G. Rücker, Richard F. Schlenk, Lars Bullinger, Hartmut Döhner, D. Gary Gilliland, Stefan Fröhling

Published in Volume 117, Issue 4

J Clin Invest. 2007; 117(4):1037–1048 doi:10.1172/JCI30182

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Figure 6

Hox gene expression in murine hematopoietic cells expressing Cdx2.

(A) As compared with cells transduced with empty vector, c-Kit⁺Lin^– murine hematopoietic progenitors expressing Cdx2 demonstrated upregulation of Hoxb8 and decreased expression of Hoxa10. There were no significant changes in mRNA levels of Hoxa6, Hoxa7, and Hoxa9. No expression was detected for Hoxb3, Hoxb6, and Hoxc6 in Cdx2-transduced cells as well as in cells transduced with empty vector. For normalization, Gapdh was used. Experiments were performed in duplicate. Values are represented as mean ± SEM. (B) Spleen cells isolated from diseased secondary BM transplant recipients demonstrated upregulation of Hoxb6 and decreased expression of Hoxa7 and Hoxa9 as compared with spleen cells obtained from age-matched control mice. There were no substantial changes in mRNA levels of Hoxa6, Hoxb8, and Hoxc6. No expression was detected for Hoxa10 and Hoxb3 in spleen cells from secondary BM transplant recipients or from control mice. The expression level of Cdx2 is also indicated. For normalization, the Gapdh gene was used. Experiments were performed in duplicate. Values are represented as mean ± SEM.