Hypoxia-inducible factor–2 (HIF-2) regulates hepatic erythropoietin in vivo
J. Clin. Invest. Erinn B. Rankin, et al. 117:1068 doi:10.1172/JCI30117 [Go to this article.]

Figure 4
Hepatic Epo expression in anemic mice is Hif-2α dependent. (A and B) Hif-1α and Hif-2α proteins are stabilized in the livers of anemic mice as determined by Western blot analysis. Nuclear protein extracts were isolated from livers of albumin-Cre mutant mice of the indicated genotypes. Nuclear protein extracts isolated from the thymus (Thy) of Vhlh-deficient mice was used as a positive Hif-1α protein control. Ponceau S staining is shown to demonstrate equal protein loading. (C–F) Real-time PCR analysis of hepatic Epo, Pgk, Bnip3, and Trf expression in anemic albumin-Cre mutant mice. White bars represent mice with hematocrit values of approximately 45%; gray bars, approximately 27%; and black bars, approximately 15%. Bars represent average mRNA transcript levels of 3 mice for each group; error bars indicate SEM. *P < 0.05, **P < 0.001 compared with control mice (Cre^–) with the same range of hematocrit as determined by Student’s t test.