Hypoxia-inducible factor–2 (HIF-2) regulates hepatic erythropoietin in vivo
J. Clin. Invest. Erinn B. Rankin, et al. 117:1068 doi:10.1172/JCI30117 [Go to this article.]

Figure 3
Differential HIF target gene expression in albumin-Vhlh/Hif-1α– and albumin-Vhlh/Hif-2α–deficient livers. Relative mRNA levels for HIF target genes in the liver of albumin-Cre mutant mice determined by real-time PCR. (A) Inactivation of Hif-2α significantly decreases the expression of HIF target genes regulating erythropoiesis (Epo) and iron transport (Trf). (B) Inactivation of Hif-1α decreases the expression of the glycolytic target gene Pgk. (C) Inactivation of both Hif-1α and Hif-2α decreases the expression of the proapoptotic gene Bnip3. Bars represent the mean mRNA transcript level of 3 mice for the albumin-Vhlh/Hif-1α/Hif-2α group and 4 mice for all other groups. Error bars represent SEM. *P < 0.05, **P < 0.001 compared with albumin-Vhlh mutants as determined by Student’s t test.