JCI - The aryl hydrocarbon receptor repressor is a putative tumor suppressor gene in multiple human cancers

The aryl hydrocarbon receptor repressor is a putative tumor suppressor gene in multiple human cancers

Enrique Zudaire, Natalia Cuesta, Vundavalli Murty, Karen Woodson, Lisa Adams, Nieves Gonzalez, Alfredo Martínez, Gopeshwar Narayan, Ilan Kirsch, Wilbur Franklin, Fred Hirsch, Michael Birrer, Frank Cuttitta

Published in Volume 118, Issue 2

J Clin Invest. 2008; 118(2):640–650 doi:10.1172/JCI30024

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Figure 4

Effect of ectopic expression of AHRR in A549.

(A) Overexpression of AHRR partially blocked expression of AHR-induced genes such as CYP1A1 (plasmid amounts expressed in μg). (B) Increased levels of AHRR in A549 resulted in reduced anchorage-dependent growth. The blue line represents A549-AHRR, and the red line represents A549-empty. Dotted lines denote standard deviation (n = 4). The maximum difference was reached 56 hours after inoculation of cells in the wells. (C) Ectopic expression of AHRR resulted in reduced anchorage-independent growth (adjacent images). Original magnification, ×10 (C); ×2 (D). (D) Representative images of tube formation assays using cocultures of A549-empty or A549-AHRR and PAE-GFP cells. A549-empty induced tube formation of PAE-GFP cells comparable to that of the internal positive control (10% FBS). Reduced tube formation was observed when PAE-GFP cells were cocultured with A549-AHRR. **P < 0.01; ***P < 0.001.