Deficiency of Src homology 2 domain–containing inositol 5-phosphatase 1 affects platelet responses and thrombus growth
J. Clin. Invest. Sonia Séverin, et al. 117:944 doi:10.1172/JCI29967 [Go to this article.]

Figure 7
Defects in MLC phosphorylation and fibrin clot retraction in SHIP1-deficient platelets. (A) MLC phosphorylation was monitored by Western blotting with an anti–phospho-MLC (Ser 19) antibody. The phosphorylation level was estimated by densitometry analysis of Western blots, and the results are expressed as percentages of the maximum phosphorylation and are mean ± SEM of 3 independent experiments. *Significant difference (P < 0.05) versus WT, according to 1-tailed Student’s t test. (B) Photographs show the extent of clot retraction in WT and SHIP1-deficient PRP samples treated with 2 IU/ml thrombin and 0.1 μg/ml atroxin. Results are representative of 2 independent experiments.