Deficiency of Src homology 2 domain–containing inositol 5-phosphatase 1 affects platelet responses and thrombus growth
J. Clin. Invest. Sonia Séverin, et al. 117:944 doi:10.1172/JCI29967 [Go to this article.]

Figure 1
Aggregation defects in SHIP1-deficient platelets. (A) Platelets were stimulated with thrombin, collagen, and the thromboxane A₂ analogue U46619, and aggregation was assessed with a Chrono-log dual-channel aggregometer under stirring at 900 rpm for 5 minutes. The profiles shown are representative of 5 independent experiments. (B) Platelets were then fixed with 1.5% paraformaldehyde for 30 minutes at room temperature, and observed by differential interference contrast with the use of a Nikon Eclipse TE 2000-U equipped with a ×10 objective and a DXM 1200 digital camera (scale bar: 40 μm). Data shown are representative of 3 independent experiments.