JCI - Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata

Ly-6C^hi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata

Filip K. Swirski, Peter Libby, Elena Aikawa, Pilar Alcaide, F. William Luscinskas, Ralph Weissleder, Mikael J. Pittet

Published in Volume 117, Issue 1

J Clin Invest. 2007; 117(1):195–205 doi:10.1172/JCI29950

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Figure 2

Peripheral blood monocytosis develops over the course of 250 days on an atherogenic diet.

(A) Number of total monocytes and Ly-6C^hi and Ly-6C^lo subtypes in bone marrow, blood, and spleens of apoE^–/– mice at various days of Western diet. Statistical analysis was based on an exponential growth curve and known cell numbers on day 0. Curve fit (solid line) and 95% confidence intervals (dashed lines) are shown. Doubling time (DT) of cell number is shown. (B) The same analysis was conducted with peripheral blood from apoE^+/+ and apoE^–/– mice that remained on chow diet. Doubling time of cell number in days is shown. (C) Splenic CD11b^hiCD90^loB220^loCD49b^loNK1.1^loLy-6G^lo cells were divided into F4/80^hiCD11c^hiI-A^b–high macrophages/dendritic cells (gate i) and F4/80^loCD11c^loI-A^b–low monocytes, which were further divided into Ly-6C^lo (gate ii) and Ly-6C^hi (gate iii) subsets. These 3 subsets were isolated and stained with HEMA 3 for microscopic analysis. Scale bar: 10 μm. Results are pooled from 8 independent experiments.