Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis
J. Clin. Invest. Hana Algül, et al. 117:1490 doi:10.1172/JCI29882 [Go to this article.]

Figure 9
Pancreas-specific ablation of RelA/p65 promotes systemic complications and multiorgan dysfunction syndrome. (A) Expression of RelA/p65 in lung tissue of rela^flox/flox and rela^Δ/Δ mice was assessed by subjecting 20 μg of lung whole cell lysates to Western blot analysis. (B–G) At the indicated times after cerulein injection, lung tissue was removed, embedded in paraffin, and stained with H&E. Higher magnification of representative H&E stains reveal marked hemorrhage and alveolar collapse in rela^Δ/Δ mice compared with rela^flox/flox mice. Original magnification, ×100 (B–E); ×200 (F and G). (H) Lung tissue of rela^flox/flox and rela^Δ/Δ mice was removed at the indicated time points and used to determine MPO enzyme activity. (I) Serum concentrations of IL-6 were determined at the time points indicated. Data represent the mean ± SD of 5 animals. *P < 0.05 versus rela^flox/flox.