Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis
J. Clin. Invest. Hana Algül, et al. 117:1490 doi:10.1172/JCI29882 [
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Figure 5Increased infiltration and TNF-α production in the pancreata of
relaΔ/Δ mice.
(
A) Mononuclear infiltration into the pancreas was visualized by immunohistochemical detection of granulocytes using a Gr-1 antibody. bv, blood vessel; ed, edema. Original magnification, ×100. (
B) Pancreatic MPO activity was measured in the pancreata of
relaflox/flox and
relaΔ/Δ mice. (
C) Infiltrating cells produced and released TNF-α. Pancreata from cerulein-treated
relaflox/flox and
relaΔ/Δ mice were stained using antibody to TNF-α. Arrowheads indicate TNF-α accumulation; asterisks indicate acinar cells. Original magnification, ×200. (
D) TNF-α levels were measured by ELISA in pancreatic cell lysates from
relaflox/flox and
relaΔ/Δ mice following 8 hourly injections of 50 μg/kg cerulein. ND, not detected. Values are mean ± SD for independent animals (
n = 4). *
P < 0.05 versus
relaflox/flox.