Evidence for tissue-resident mesenchymal stem cells in human adult lung from studies of transplanted allografts
J. Clin. Invest. Vibha N. Lama, et al. 117:989 doi:10.1172/JCI29713 [
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Figure 4MSCs isolated from BAL fluid of transplanted lungs are of donor sex genotype. FISH of mesenchymal cells isolated from BAL fluid of sex-mismatched lung transplant recipients was performed using CEP X Spectrum Orange and Y Spectrum Green DNA probes to distinguish cells of donor versus recipient origin. The top row shows the male sex chromosome status of mesenchymal cells obtained from BAL of a male donor lung transplanted into a female host. Red signal indicates X chromosome, and green signal indicates Y chromosome. Cells were scored in interphase (left). A cell in metaphase demonstrating specificity of the DNA probes is shown on the right. Bottom panels demonstrate the female sex chromosome status of mesenchymal cells obtained from BAL of a female donor lung transplanted into a male recipient (left: interphase cells; right: representative cell in metaphase).
