Myeloid progenitors differentiate into microglia and promote vascular repair in a model of ischemic retinopathy
J. Clin. Invest. Matthew R. Ritter, et al. 116:3266 doi:10.1172/JCI29683 [Go to this article.]

Figure 3
The CD44^hi subpopulation expresses markers and genes characteristic of myeloid cells. Two-color flow cytometry was used to characterize CD44 populations in whole mouse BM. All cells were labeled with an antibody against CD44 and colabeled with the indicated antibodies. CD44^hi cells are distinguished from CD44^lo and CD44^– cells by the horizontal lines in each plot. The CD44^hi population showed strong labeling for CD11b and Ly6G/C. Fractions of CD44^hi cells (to the right of the vertical lines) were positive for F4/80, CD14, and CD115. These antigens are present on cells of the myeloid lineage (38–42). Positive labeling for CD11a and cKit was also observed on CD44^hi cells. CD44^lo cells labeled strongly with Ter119 and CD45R/B220 which are markers for erythroid and lymphoid cells, respectively (43, 44).