Myeloid progenitors differentiate into microglia and promote vascular repair in a model of ischemic retinopathy
J. Clin. Invest. Matthew R. Ritter, et al. 116:3266 doi:10.1172/JCI29683 [Go to this article.]

Figure 2
BM subpopulations accelerate retinal revascularization and reduce preretinal neovascular tuft formation in OIR. (A–D) Computer-assisted image analysis was used to calculate the area of retinal vessel obliteration (yellow) and preretinal neovascular tuft formation (red) in whole mounts from OIR eyes at P17 (17). (E) Retinas treated with Lin^–HSC prior to hyperoxia showed an almost 6-fold reduction in obliterated area versus uninjected controls and an approximately 5-fold reduction compared with vehicle-treated controls. (F) Lin^–HSC treatment significantly reduced neovascular tufts compared with uninjected and vehicle-treated eyes. (G) Lin^–HSCs reduced obliteration when administered prior to hyperoxia and during hyperoxia (P9 or P11) or just after return to normoxia (P12). inj, injected. (H) Mouse BM contained CD44^hi and CD44^lo fractions and the Lin^–HSC population was enriched for CD44^hi cells (red). Insets: Light-scattering properties of the CD44^hi cells were typical of monocytes and granulocytes, while those of CD44^lo cells were typical of lymphocytes. (I and J) Representative P17 retinas from eyes treated with CD44^lo and CD44^hi BM cells at P7. (K and L) Areas of obliteration (yellow) and neovascularization (red) at P17. When treated at P7, vascular obliteration (M) and neovascularization (N) were reduced in eyes treated with CD44^hi cells with efficacy similar to eyes treated with Lin^–HSC cells. Obliteration was similar between eyes treated with Lin^–HSC and CD44^hi cells, and neovascularization did not differ significantly between these groups (P = 0.25). Values represent mean ± SEM. *P < 10^–5; **P ≤ 0.006. Magnification, ×4.