Adiponectin deficiency increases leukocyte-endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo
J. Clin. Invest. Raogo Ouedraogo, et al. 117:1718 doi:10.1172/JCI29623 [
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Figure 4Functional eNOS activity is necessary for the antiinflammatory effect of gAd. Superfusion of the mouse mesenteric microcirculation with the eNOS inhibitor
l-NAME increases leukocyte adhesion in WT mice (white bars). Two 25 μg doses of gAd given 24 hours and 2.5 hours before intravital microscopy failed to prevent leukocyte adhesion in response to
l-NAME. Moreover, attenuation of leukocyte adhesion by administration of gAd (25 μg/twice daily for 6 consecutive days) to Ad
–/– mice is acutely hampered by superfusion of the microcirculation with
l-NAME (black bars). Data are expressed as the mean ± SEM. Numbers at the base of the bars indicate the number of mice studied in each group.
