Trichostatin A increases SMN expression and survival in a mouse model of spinal muscular atrophy
J. Clin. Invest. Amy M. Avila, et al. 117:659 doi:10.1172/JCI29562 [Go to this article.]

Figure 5
TSA treatment increases snRNP assembly activity in brains of SMA mice. (A) snRNP assembly reactions were carried out using in vitro transcribed radioactive U1 snRNA and 25 mg of brain extracts from either vehicle- or TSA-treated SMA mice. Following immunoprecipitation with anti-Sm antibodies, input (2.5%) and immunoprecipitated U1 snRNAs were analyzed by electrophoresis on 10% polyacrylamide, 8M urea denaturing gels and autoradiography. (B) Quantification of relative snRNP assembly activity in brain extracts from vehicle- and TSA-treated SMA mice. Brain extracts from either vehicle-treated (n = 5) or TSA-treated (n = 7) SMA mice were prepared and analyzed by snRNP assembly and immunoprecipitation experiments as in A, and the amount of immunoprecipitated U1 snRNA was quantified as described in Methods. Values represent mean ± SEM. *P < 0.01.