Trichostatin A increases SMN expression and survival in a mouse model of spinal muscular atrophy
J. Clin. Invest. Amy M. Avila, et al. 117:659 doi:10.1172/JCI29562 [Go to this article.]

Figure 4
Repeated doses of TSA increase SMN+7 mRNA levels and SMN protein levels in SMA mice. (A) SMN+7, SMNΔ7, follistatin, and BDNF mRNA levels and (B and C) SMN and BDNF protein levels were determined in brain, liver, spinal cord, and muscle isolated 4 hours after the last dose from SMA mice treated daily from P5 through P13. (B) Western blots, with each lane representing 1 animal. In the upper panels, the upper bands show full-length SMN protein; the lower bands show SMNΔ7 protein. (C) Quantification of Western blots shown in B. Lanes denoted with arrowheads were excluded as underloaded relative to the other visualized lanes. SMN protein bands were not visible in the muscle of untreated SMA mice, although they were evident in treated mice; therefore, quantification of the changes in muscle SMN protein levels was limited. Values represent mean ± SEM. ^#P < 0.05; *P < 0.01; **P < 0.001.