Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation
J. Clin. Invest. Alexander Zarbock, et al. 116:3211 doi:10.1172/JCI29499 [
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Figure 3Platelet P-selectin plays key role in the development of ALI. (
A–
C) Injecting P-selectin Abs 15 minutes after the induction of ALI (
n = 4–5 mice per group) improved gas exchange (
A) and reduced interstitial (data not shown) and BAL fluid (
B) PMNs as well as permeability (
C). (
D–
G) To determine whether hematopoietic (platelet) or nonhematopoietic (endothelial) P-selectin is responsible for neutrophil recruitment in ALI, BM chimeras (WT into WT; WT into
Selp–/–;
Selp–/– into WT;
Selp–/– into
Selp–/–) were tested (
n = 4–5 mice per group). Mice lacking platelet P-selectin showed improved gas exchange (
D), reduced PMN accumulation in the intravascular (
E), interstitial (data not shown), and alveolar compartments (
F), and diminished permeability (
G) compared with mice expressing hematopoietic P-selectin.
#P < 0.05;
ΧP < 0.05 versus platelet
Selp–/–.
